What Are Coliform Bacteria? What a Positive Well Test Means

Coliform bacteria

A broad group of bacteria used as an indicator: most are harmless, but finding them in water means germs from soil or waste have a way in.

Total coliformFecal coliformE. coli
  1. Total coliform: a broad group found naturally in soil, on vegetation and in surface water. Most don't cause illness; a positive result means there's a path for contamination into the water.
  2. Fecal coliform: the part of that group that lives in the intestines of warm-blooded animals.
  3. E. coli: a fecal coliform that points to human or animal waste reaching the water, the most serious of the three results.
The three results on a well test, from broadest to most specific. Only the inner two point to fecal contamination.

At a glance

Found in
Soil, vegetation and surface water; fecal coliforms in animal intestines
What it signals
A pathway from a source of bacteria into the water supply
Most serious result
E. coli, a sign of human or animal waste
Test
Every year for a private well, from a state-certified lab
Typical cost
About $10 to $30 (Penn State Extension)

Coliform bacteria are the first thing a well test looks for, not because they make you sick, but because they show whether germs can get into your water.

What does “coliform present” mean?

Coliforms are a large group of bacteria that occur naturally in soil, on vegetation and in surface water, according to the Wisconsin DNR. The Minnesota Department of Health notes that most don’t cause disease, but their presence suggests disease-causing microorganisms may be there too. Penn State Extension puts it as evidence that “a contamination pathway exists” between a source such as surface water, a septic system or animal waste and the water supply.

Lab reports often split the result into three levels:

  • Total coliform: the whole group, the indicator.
  • Fecal coliform: the coliforms that live in the intestines of warm-blooded animals.
  • E. coli: a specific fecal coliform. Penn State calls a positive E. coli result much more serious than coliform alone, because it means human or animal waste is reaching the water.

What should you do about it?

On a private well, it’s your job. The EPA recommends testing for total coliform every year, and after flooding, well repairs or any change in taste, color or smell. If a test comes back positive, use boiled or bottled water, retest, and look for how the bacteria got in; our guide to bacteria in well water walks through retesting, shock chlorination and when UV makes sense.

Published by the Checked Water editorial team

Sources

  1. Coliform Bacteria (Clinical guideline, Penn State Extension, 2025)
  2. Bacterial Safety of Well Water (Clinical guideline, Minnesota Department of Health, 2025)
  3. Bacteriological Contamination of Drinking Water Wells (PUB-DG-003-2017) (Clinical guideline, Wisconsin Department of Natural Resources, 2017)
  4. Protect Your Home's Water (Clinical guideline, US EPA, 2026)

Where Coliform bacteria comes up